selected clone meaning in English
入选无性系
Examples
- At the outset they must contain several ramets of every selected clone .
在开始时每个入选无性系必须包括几个无性繁殖后代。 - [ results ] all the selected clones gave strong signal in dot bloting and elisa
[结果]对肽库进行三轮筛选后，噬菌体克隆具有良好的富集效果。 - By sequencing random - selected clones from the library and using bioinformatics , 54 novel expressed sequence tags ( ests ) were obtained , in which there were 14 full - length cdnas , including peroxiredoxin gene , hmg - box gene , ubiquitin gene , signal recognition particle , acetyltransferase gene and lysozyme gene
有14个克隆包含有完整的开放阅读框，其中包括抗氧化物酶基因prx 、 dna结合蛋白基因、泛素基因、信号识别蛋白基因、溶菌酶基因和乙酰基转移酶基因等具有重要生物学功能的基因。 - Thirdly , the hyl gene was cloned to puc19 and pbr322 respectively . then the fragment containing ampr derived from puc19 and hasa came from pse380 - has were inserted into hyl either or both in vitro . transformed the recombinated vectors into s . equi by electro - operation , then plating on hag solid medium containing ampicillin , and selected clones hi which the hyl gene was inactivated by gene replacement through homologous recombination
( 1 )直接插入外源基因灭活hyl方法：将hyl片段与载体puc19 pbr322连接后，用来自puc19的amp ~ r抗性基因片段从hyl基因的中部插入将载体上的hyl片段分为两部分； ( 2 )用hasa替换hyl部分片段的方法：将hyl片段与载体pucl9 pbr322连接后，用hasa片段替换部分hyl ，再将amp ~ r片段接入到hasa上游。